Droplet Single-Cell Cut&Tag Kit – 8rxn

Single-Cell CUT&Tag is an innovative assay that precisely measures epigenetic modifications in individual cells by targeting histone post-translational modifications (PTMs) or transcription factors (TFs). Unlike multiomic approaches that capture RNA, Single-Cell CUT&Tag focuses solely on tagmented DNA, allowing for deeper coverage of epigenetic marks without the complexity of concurrent transcriptomic profiling. This method is especially suited for studies in which RNA is either degraded (e.g., FFPE samples) or not required, including cell differentiation assays or drug-treatment experiments in cell lines. By integrating seamlessly with single-cell barcoding platforms — such as 10x Chromium® (ATAC) or Fluent PiP-seq® — Single-Cell CUT&Tag provides a high-resolution snapshot of gene regulatory states in each cell, driving new insights into how epigenetic changes shape biological responses.

Key Advantages

• ✅ Platform Compatibility: Utilize 10X Chromium or Fluent PiP-seq
• ✅ Flexible Profiling: Targets histone PTMs, TFs, or remodelers
• ✅ High Sensitivity & Resolution: Single-cell resolution without sorting
• ✅ Streamlined Workflow: Pre-optimized for leading single-cell systems

Application

Enhance your precision medicine research with the scCut&Tag Droplet - 8 Reaction Kit from Epigenome Technology. Bringing Cut&Tag epigenetic and TF profiling to single-cell resolution, this kit is compatible with the 10X Genomics droplet platform and allows you to profile histones, protein complexes, and TFs at an unprecedented single-cell level. Each kit provides up to 8 reactions, enabling analysis of up to 80,000 recovered cells, making it ideal for studying active marks or conducting well-characterized cellular experiments. Please note, the procedure requires a 10X Genomics ATAC kit.

Performance

• Recovery: 10,000+ cells per reaction
• UMI counts: 5000+ for broad marks, 1000+ for narrow marks
• Compatibility: fresh, frozen, and cryopreserved samples
• Accuracy: Highly Correlated (>98%) with matched bulk CUT&Tag

What You Need to Get Started

• Antibody: Validated primary antibody for target of interest
• Single-Cell Platform: 10x Genomics Chromium (ATAC) or Fluent PiP-seq system
• Reagents: 10x Genomics or Fluent Bio reagents for cell processing
• Sequencing: Illumina-compatible sequencing system