Epigenome Single-Cell CUT&Tag Kit (10x-Compatible)

Chromatin profiling at single-cell resolution using 10x Genomics ATAC platform

Epigenome Technologies’ Single-Cell CUT&Tag Kit enables high-resolution mapping of histone modifications or transcription factor binding at the single-cell level using the widely adopted 10x Genomics ATAC-seq platform. This kit transforms the ATAC pipeline into a powerful epigenetic profiling tool without requiring RNA or chromatin accessibility data.

Epigenome Technologies, Inc. is a biotech company focused on developing next-generation single-cell multiomic tools for high-resolution analysis of epigenetic regulation and gene expression. Originating from the lab of epigenomics pioneer Dr. Bing Ren, the company uses proprietary technology to decode gene regulation with single-cell precision. Its products and services include kits for simultaneous single-cell CUT&Tag and RNA sequencing from the same cell.

Key Advantages

• Dual-omic Detection
  Simultaneous profiling of histone modifications or transcription factor binding and RNA expression from the same cell.
• Multiplexing Capability
  Pool multiple samples into a single 10x Multiome lane—cutting costs while maintaining data quality.
• Optimized for Droplet Systems
  Seamlessly integrates with 10x Genomics workflows for scalable, high-throughput analysis.
• Low Input, High Sensitivity
  Detect subtle regulatory changes from as few as 50,000 cells per sample.
• Flexible and Scalable
  Compatible with various histone marks, TFs, and sample types—from primary tissues to cultured cells.

Application

• Cost-Efficient Complex Disease Modeling
  Study cancer, neurodegeneration, or autoimmune disorders across multiple conditions or time points in one run.
• Drug Discovery & Toxicity Screening
  Assess regulatory response and gene expression changes across treated vs. control samples in parallel.
• Rare Cell Population Analysis
  Reveal regulatory dynamics in rare or mixed cell populations without needing cell sorting.
• Biomarker Discovery
  Identify multiomic signatures linked to disease states, therapy response, or resistance.

Performance

• Recovery: 10,000+ cells per reaction
• UMI counts: 5000+ for broad marks, 1000+ for narrow marks
• Compatibility: fresh, frozen, and cryopreserved samples
• Accuracy: Highly Correlated (>98%) with matched bulk CUT&Tag

What You Need to Get Started

• Antibody: Validated primary antibody for target of interest
• Single-Cell Platform: 10x Genomics Chromium (ATAC) or Fluent PiP-seq system
• Reagents: 10x Genomics or Fluent Bio reagents for cell processing
• Sequencing: Illumina-compatible sequencing system